32 kda alpha subunit  (Bio-Rad)

Journal: Molecular endocrinology (Baltimore, Md.)

Article Title: N-linked oligosaccharides direct the differential assembly and secretion of inhibin alpha- and betaA-subunit dimers.

doi: 10.1210/me.2007-0050

Figure Lengend Snippet: Fig. 2. Inhibin - and A-Subunit Products Secreted from CHO Cells Medium from inhibin-expressing CHO cells was TCA precipitated and examined by immunoblot analysis with the inhibin -subunit antibody (A) or the inhibin A-subunit antibody (B). C, Effect of tunicamycin on inhibin A secretion. CHO cells expressing inhibin A were incubated with vehicle (lanes 1 and 2) or 2.5 g/ml tunicamycin (lanes 3 and 4) and metabolically labeled. Medium was immunoprecipitated (IP) with anti--subunit (lanes 1 and 3) or anti-A-subunit antibody (lanes 2 and 4) and subjected to SDS-PAGE and autoradiography. *, Nonspecific nonglycosylated protein that immunoprecipitated only in tunicamycin-treated samples.

Article Snippet: Immunoblot analysis was performed using the mouse antihuman inhibin -subunit antibody that recognizes amino acids 1–32 of the inhibin -subunit (Serotec, Raleigh, NC) or a polyclonal antibody specific for the inhibin A-subunit (provided by Dr. W. Vale, The Salk Institute, La Jolla, CA), followed by the appropriate secondary antibody conjugated to horseradish peroxidase (GE Healthcare, Little Chalfont, UK), and detected using ECL plus (GE Healthcare).

Techniques: Expressing, Western Blot, Incubation, Metabolic Labelling, Labeling, Immunoprecipitation, SDS Page, Autoradiography